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US 4,282,002 AGranted
Sensitized sheep stroma immunoassay for rheumatoid factor
AssigneeAkzona Incorporated
InventorRita C. Prodell
Life of this patent
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AssigneeAkzona Incorporated
InventorRita C. Prodell
Estimating the term…
A new and immunochemically useful reagent is disclosed for the detection of rheumatoid factors (RF) in human blood, plasma, serum or synovial fluid in agglutination reactions, together with a new clinical diagnostic test and test slide using said reagent. The reagent consists essentially of a supply of red cell stroma having absorbed upon their surface a sensitizing agent. Preferably the red cell stroma is sheep red cell stroma, and most preferably the sensitizing agent is rabbit anti-sheep red cell globulin.
A reagent for the detection of rheumatoid factors in human blood, plasma, serum or synovial fluid by immunochemical reaction with the factors, consisting essentially of a supply of red cell stroma having adsorbed upon their surface a sensitizing agent, which sensitizing agent sensitizes the red cell stroma for the detection of said rheumatoid factors.
A test slide adapted for the performance on the surface thereof of an immunochemical test for detection of rheumatoid factors in human blood, plasma, serum or synovial fluid, comprising:
A method for preparing sensitized red cell stroma, the resulting sensitized stroma providing a reagent for detecting rheumatoid factors in human blood, plasma, serum or synovial fluid by immunochemical agglutination, said method comprising the steps of:
The method for detection or determination of rheumatoid factors in human blood, plasma, serum or synovial fluid by means of the immunochemical reaction of said factors, which method comprises:
Dependencies are inferred from the claim text.
No related US applications are recorded in the indexed filing history.
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Immunoassay; Biospecific binding assay; Materials therefor: for pre-existing immune complex or autoimmune disease , i.e. systemic lupus…
Diffuse connective tissue disease, e.g. Sjögren, Wegener's granulomatosis: arthritis; Rheumatoid arthritis, i.e. inflammation of peripheral joints
Chemistry: analytical and immunological testing: additives, e.g. buffers, diluents, preservatives
Investigating or analysing materials by determining their chemical or physical properties
Term ended 6 Sep 1999 (estimated)
USPTO file history isn’t available for this patent, so only indexed dates are shown.
1. Field of the Invention
This invention relates to a new immunochemical reagent, a new clinical diagnostic test using such reagent, and a test slide using such reagent. More particularly, the invention relates to a new carrier on which globulins useful in clinical diagnostic agglutination reactions may be permanently fixed. The new reagent is particularly useful in agglutination reactions for the detection of rheumatoid factors (RF), and may be advantageously dried onto a test slide to which a test solution may be later applied.
2. Description of the Prior Art
The purpose of this example is to examine the utility of sensitizing fresh sheep cells with various available antibodies after the cells had been prepared by collecting them fresh in citrate solution, washing them 2 times in 0.9% NaCl solution, and then resuspending to 4% cell concentration in either borate buffer or 0.9% NaCl solution. The cells were then processed in the following various ways:
1. In the first case, to 200 ml of sheep cells in saline was added 5.0 ml of FII gamma globulin solution (100 mg. % in 0.9% sodium chloride solution) that had been prepared and held frozen until ready for use. The cells were incubated in a glass container for 7 days at either 2.degree.-8.degree. in a refrigerator or 37.degree. C. in a warm air incubator after which they were washed in 0.9% NaCl and resuspended to the original volume in borate buffer and then incubated in a glass container put into a warm air incubator at 37.degree. C. until stroma formed.
2. In the second case, 100 ml of the cells prepared in saline were used and to them was added 100 ml. of a 1:37.5 dilution of rabbit anti-sheep serum preparation. Incubation and stroma formation were as in 1.