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US 4,282,001 AGranted
Compositions and method for reducing turbidity in samples
AssigneeBoehringer Mannheim GmbH
InventorsSigmar Klose, Herbert Buschek, Helmut Schlumberger
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AssigneeBoehringer Mannheim GmbH
InventorsSigmar Klose, Herbert Buschek, Helmut Schlumberger
Estimating the term…
For the removal of turbidities in reaction mixtures clouded by sample material, especially for photometric measurement, there are added to the reaction mixture: (a) at least one low molecular weight organic compound, such as an aromatic alcohol or amine, which contains an electronegative substituent, for example a phenol or naphthol, which is substituted with one or more halogen atoms and/or hydroxyl groups and (b) at least one detergent which dissolves to give without compound (a), a clear solution and which forms a sparingly soluble complex with component (a), such as a non-ionic polyethylene oxide adduct or a cationic detergent, especially an alkyl, aralkyl or alkylthio ether or an alkyl or aralkyl ester of a polyethylene oxide glycol containing 8 to 22 carbon atoms in the alkyl radical and 5 to 25 ethylene oxide units or an alkyl dimethyl benzyl ammonium chloride compound containing 8 to 22 carbon atoms in the alkyl radical, in an amount sufficient for the appearance of a precipitate of the complex, whereafter further detergent is added thereto until the precipitated complex dissolves again.
Process for the removal of turbidity in aqueous reaction mixtures clouded by sample material, which method comprises adding to the reaction mixture
Composition for removing turbidity from serum or plasma-clouded reaction mixtures which composition comprises
Dependencies are inferred from the claim text.
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Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing:…
Measuring or testing processes involving enzymes, nucleic acids or microorganisms ; Compositions therefor; Processes of preparing such…
Measuring or testing processes involving enzymes, nucleic acids or microorganisms ; Compositions therefor; Processes of preparing such…
Measuring or testing processes involving enzymes, nucleic acids or microorganisms ; Compositions therefor; Processes of preparing such…
Term ended 4 Apr 1999 (estimated)
USPTO file history isn’t available for this patent, so only indexed dates are shown.
The present invention is concerned with a process and a reagent for the removal of turbidities in sample materials, such as biological sample materials.
Reagents to remove turbidity are useful in samples, e.g., serum or plasma-clouded reaction mixtures which are, inter alia, employed in clinical-chemical analysis and foodstuff analysis, as well as in water and effluent analysis. In order to achieve a higher specificity, enzymes are thereby frequently used.
A known problem in photometric analysis is the influence of turbidities of the sample, such as serum or plasma, on the analysis results when the measurement is carried out without sample blank value. The avoidance of the measurement of a sample blank value is desired in order to reduce the reagent consumption, to simplify the measurement and evaluation procedure and to reduce the amount of sample needed. In the case of the use of one-pot reagents, i.e. those containing a mixture of all the reaction components, in principle, the measurement of a sample blank value is even impossible. Such turbidities have various causes, a frequent cause being a high triglyceride content.
The following Examples are given for the purpose of illustrating the present invention:
The following combinations according to the present invention gave, within 1 minute, up to a maximum of 2 hours, a removal of the turbidity in the system. 2 ml. test mixture +20 .mu.l drinking milk with 3.5% fat content.
1 cm. optical path length 546 nm; 25.degree. C.