Easily split substrates for the quantification of proteases

Abstract

Easily split enzyme substrates for the quantification of proteases having the general formula R.sub.1 --p-Glu--A.sub.1 --A.sub.2 --NH--R.sub.2, where R.sub.1 =H or a protective group, preferably t-butyloxycarbonyl, benzyloxycarbonyl; A.sub.1 =Gly, Ala, Val, Leu, Ile, Ser, Thr, Pro, Pip, Phe or Tyr; A.sub.2 =Arg or Lys; R.sub.2 =an aromatic, possibly substituted, hydrocarbon group, wherein --NH--R.sub.2 is a physico-chemically determinable group, preferably a chromogenic or fluorogenic group which is split by a present enzyme and then forms a cleavage product of the formula H.sub.2 N--R.sub.2 the amount of which can be quantified. Processes for the production of said substrates. Method in the laboratory diagnostics of proteases using said substrates.

Dependencies are inferred from the claim text.