Cellulase-producing microorganism
AssigneeThe United States of America as represented by the Secretary of the Army
InventorBenedict J. Gallo
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Abstract
A process and a microorganism for synthesizing cellulase enzymes are described. The microorganism is a mutant strain of an Ascomycete fungus capable of synthesizing cellulases. The synthesis of cellulases by the mutant is nonrepressed by glycerol, repressed by glucose but not subject to postrepression lag, and inducible to high levels by lactose. Furthermore, the combination of lactose with xylose elicits maximum cellulase synthesis by the mutant.
Claims7
A biologically pure culture of Trichoderma reesei strain MCG77, said culture having the capability to synthesize cellulase enzymes, the synthesis of the cellulases being nonrepressed by glycerol, repressed by glucose but not subject to post repression lag, and inducible by lactose, the lactose induction being potentiated by xylose, whereby maximum cellulose synthesis is elicited by the presence of lactose and xylose in combination in the culture medium, the organism being genetically haploid under laboratory culture conditions.
A process for producing enzymes capable of catalyzing the hydrolysis of cellulose comprising:
- inoculating a growth medium with a microorganism comprising a mutant strain of Trichoderma reesei having the capability to synthesize cellulase enzymes, the synthesis of the cellulase enzymes being nonrepressed by glycerol, repressed by glucose but not subject to post repression lag, and inducible by lactose, the lactose induction being potentiated by xylose, and inducible by cellulose, the cellulose induction being potentiated by xylose, said growth medium containing an inducer of cellulase synthesis, and
- incubating the microorganism for a time sufficient to obtain cellulase synthesis.
Dependencies are inferred from the claim text.