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US 4,272,523 AGranted
Fractionating citrate-stabilized plasma
AssigneeBiotest-Serum-Institut GmbH
InventorsRonald Kotitschke, Wolfgang Stephan
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AssigneeBiotest-Serum-Institut GmbH
InventorsRonald Kotitschke, Wolfgang Stephan
Estimating the term…
The present invention relates to a method for making fibrinogen, a prothrombin complex containing the coagulation factors II, VII, IX and X that can contain antithrombin III, antithrombin III and a solution of stable serum proteins from a blood plasma stabilized with citrate which is characterized in that from the plasma, by adsorption on colloidal silica of a specific surface of 50 to 400 m.sup.2 /g and a concentration of 50 to 400 mg per g plasma protein, fibrinogen is isolated; and in that thereupon (a) citrate and calcium ions are removed by ultrafiltration or dialysis and then from the protein solution, over anion exchangers or tricalcium phosphate that adsorb proteins, the coagulation factors II, VII, IX and X and antithrombin III are adsorbed, or (b) the coagulation factors II, VII, IX and X are adsorbed prior to the ultrafiltration or dialysis, antithrombin III then not being simultaneously adsorbed, and antithrombin III is adsorbed on the said adsorbents after the removal of the citrate and calcium ions by ultrafiltration or dialysis; and in that thereupon from the remaining plasma fluid further unstable proteins are removed by another adsorption on colloidal silica and a solution of stable serum proteins is obtained.
A method for obtaining fibrinogen, a prothrombin complex containing coagulation factors II, VII, IX and X, antithrombin III, and a solution of stable serum proteins from a citrate-stabilized blood plasma comprising contacting the plasma with colloidal silica of a specific surface of about 50 to 400 m.sup.2 /g, about 50 to 400 mg of silica being employed per g of plasma protein, thereby to adsorb the fibrinogen from the plasma, separating the silica and adsorbed fibrinogen from the residual protein solution, subjecting the residual protein solution to ultrafiltration or dialysis so as to remove citrate and calcium ions therefrom, contacting the residual protein solution with an adsorbent for the prothrombin complex and antithrombin III, and separating the adsorbent with material adsorbed thereon from the residual solution of stable serum proteins.
A method for obtaining fibrinogen, a prothrombin complex containing coagulation factors II, VII, IX and X, antithrombin III, and a solution of stable serum proteins from a citrate-stabilized blood plasma comprising contacting the plasma with a first selective adsorbent for coagulation factors II, VII, IX and X but not for antithrombin III, separating the first adsorbent from the liquid, contacting the liquid with colloidal silica of a specific surface of about 50 to 400 m.sup.2 /g, about 50 to 400 mg of silica being employed per g of protein thereby to adsorb the fibrinogen, subjecting the liquid to ultra-filtration or dialysis so as to remove citrate and calcium ions therefrom, and contacting the liquid with a second selective adsorbent to adsorb the antithrombin III and leave a solution of stable serum proteins.
A method for obtaining fibrinogen, a prothrombin complex containing coagulation factors II, VII, IX and X, antithrombin III, and a solution of stable serum proteins from a citrate-stabilized blood plasma comprising contacting the plasma with colloidal silica of a specific surface of about 50 to 400 m.sup.2 /g, about 50 to 400 mg of silica being employed per g of plasma protein, thereby to adsorb the fibrinogen from the plasma, separating the silica and adsorbed fibrinogen from the residual protein solution, contacting the residual protein solution with an anion exchanger thereby to remove citrate from the solution, separating the anion exchanger and adsorbed prothrombin complex from the residual solution, and subjecting the residual solution to ultrafiltration or dialysis to remove calcium ions therefrom.
Dependencies are inferred from the claim text.