Enzyme immobilization
AssigneeNovo Industri A/S
InventorSven Branner-Jorgensen
Life of this patent
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Abstract
An immobilized enzyme granular product suitable for use in expanded and fluidized bed operations is formed by gelatine coating a dense particulate material, e.g., a mineral oxide, and hardening the coating with glutaraldehyde, then treating the coated particles with a pasty mixture of an enzyme substance and polyethylene imine, and thereafter treating the mixture with glutaraldehyde, followed by granulating and drying. Sand and titanium dioxide are preferred particulate materials. Lactase, urease, and inulinase are preferred enzymes, preferably in the form of whole or homogenized microbial cells.
Claims21
Process for preparation of an immobilized enzyme product which comprises:
- coating a particulate, dense material with gelatine then treating the so-coated material of particle size between about 0.1 and 1 mm with glutaraldehyde;
- preparing a paste comprising an enzyme product and an aqueous polyethylene imine solution;
- mixing the glutaraldehyde treated particulate material with the paste;
- thereafter treating the resulting pasty mixture with glutaraldehyde to form a mass;
- and
- granulating and drying the said mass.
Dependencies are inferred from the claim text.