Back to results
US 4,256,725 AGranted
Preparation of solid substrate containing receptor and labeled form of ligand for assays
AssigneeBecton, Dickinson and Company
InventorsHerman Rutner, Thomas F. Dodd
Life of this patent
Estimating the term…
Abstract
A solid substrate is simultaneously contacted with a labeled form of a ligand to be assayed, a receptor for the ligand to be assayed and a solution of an ionic salt to produce a solid substrate which contains the labeled form of the ligand and the receptor. In a subsequent assay for the ligand, the solid substrate is contacted with a sample containing the ligand, whereby the labeled form of the ligand is available for equilibration with the receptor in competition with the ligand to be assayed.
Claims20
A process for preparing an article suitable for use in a solid phase assay, comprising:
- contacting a solid substrate with a mixture of the labeled form of a ligand to be assayed, a receptor for the ligand and a solution of an ionic salt having an ionic strength of at least 0.5, and no greater than 10.0 whereby the receptor and labeled form of the ligand are placed on the solid substrate, said labeled form of the ligand being reversibly placed on the solid substrate, whereby during an assay for the ligand, the labeled form of the ligand is available for equilibration with the receptor in competition with the ligand to be assayed.
Dependencies are inferred from the claim text.