Determination of hydrogen peroxide
AssigneeWako Pure Chemical Industries, Ltd.
InventorsKazuhiko Yamanisi, Kuniaki Tokuda, Tosiro Hanada
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Abstract
Colorimetrical determination of hydrogen peroxide in clinical examinations, food additives analyses and general analyses can be carried out accurately, rapidly and economically by using a composition for producing color producing reagents comprising 4-aminoantipyrine, a N-substituted-3-alkylaniline and a hydrogen peroxide activating agent such as peroxidase, said composition forming a coloring material having excellent sensitivity and color stability in contact with hydrogen peroxide.
Claims10
A composition for producing color producing reagents for determining hydrogen peroxide which comprises
- 4-aminoantipyrine,
- a N-substituted-3-alkylaniline of the formula:
- ##STR4## wherein R.sup.1 is a member selected from the group consisting of a lower alkyl group having 1 to 5 carbon atoms and a hydroxyalkyl group having 1 to 5 carbon atoms;
- R.sup.2 is a member selected from the group consisting of a lower alkyl group having 1 to 5 carbon atoms and a hydroxyalkyl group having 1 to 5 carbon atoms and R.sup.3 is methyl, ethyl or propyl, and an agent for activating hydrogen peroxide.
In a method for determining hydrogen peroxide in an enzymatic reaction system wherein 4-aminoantipyrine is oxidatively condensed with an aniline to produce a color developed material and the optical absorbance of the color developed material is determined, the improvement comprising employing an N-substituted-3-alkylaniline of the formula:
- ##STR5## wherein R.sup.1 is a member selected from the group consisting of a lower alkyl group having 1 to 5 carbon atoms and a hydroxyalkyl group having 1 to B 5 carbon atoms;
- R.sup.2 is a member selected from the group consisting of a lower alkyl group having 1 to 5 carbon atoms and a hydroxyalkyl group having 1 to 5 carbon atoms and R.sup.3 is methyl, ethyl or propyl, in the presence of an agent for activating hydrogen peroxide.
Dependencies are inferred from the claim text.