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AssigneeSumitomo Chemical Company, Limited
InventorsHideo Hirohara, Shigeyasu Nabeshima, Masanori Fujimoto, Tsuneyuki Nagase
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Enzymes are immobilized by a process wherein the carrier used for immobilizing is a hydrophilic pullulan gel having a three-dimensionally reticulated structure obtained by the reaction between pullulan and a bifunctional compound capable of forming an ether linkage with the hydroxyl group contained in the glucose unit of pullulan, or an ionic pullulan gel obtained by the reaction between said hydrophilic pullulan gel and a compound having at one end an ionizable group and at the other end a functional group capable of forming an ether linkage with the hydroxyl group in the pullulan gel in the presence of an alkaline coumpound. Immobilized enzymes prepared according to this process have a high activity and good retention of the activity.
An immobilized enzyme prepared by a process which comprises covalently bonding an enzyme on an enzyme-immobilizing carrier by utilizing the reactivity of hydroxyl groups in said enzyme-immobilizing carrier which is a hydrophilic pullulan gel in spherical bead form of 10 to 500.mu. in diameter having a water regain of 1 to 50 g/g which is obtained by crosslinking pullulan with epichlorohydrin.
A process for preparing an immobilized enzyme which comprises covalently bonding an enzyme on an enzyme-immobilizing carrier by utilizing the reactivity of hydroxyl groups in said enzyme-immobilizing carrier which is a hydrophilic pullulan gel in spherical bead form of 10 to 500.mu. in diameter having a water regain of 1 to 50 g/g which is obtained by crosslinking pullulan with epichlorohydrin.
Dependencies are inferred from the claim text.