Process for the manufacture of pyruvate oxidase, and analytical method and kit for the use of the same
AssigneeToyo Jozo Kabushiki Kaisha
InventorsHideo Misaki, Kazuo Matsuura, Saburo Harada, Satoshi Takenaka, Yoshifumi Horiuchi
Life of this patent
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Abstract
Pyruvate oxidase can be produced by culturing Pediococcus sp. B-0667, Streptococcus sp. B-0668, Aerococcus viridans IFO-12219 or Aerococcus viridans IFO-12317. It is useful for analysis for pyruvic acid, because it catalyzes the reaction of pyruvic acid, phosphate and oxygen to form acetylphosphate, carbon dioxide and hydrogen peroxide. A kit containing the various reagents for such analysis is also provided by this invention.
Claims10
A process for the manufacture of pyruvate oxidase, which comprises culturing a pyruvate oxidase-producing microorganism belonging to a genus selected from Pediococcus, Streptococcus and Aerococcus, in a nutrient culture medium, and separating the pyruvate oxidase thus produced from the culture medium.
A method for the analysis of pyruvic acid in a sample containing pyruvic acid or a pyruvate-liberating system, comprising contacting said sample with pyruvate oxidase and measuring a consumed component or a generated component, in which said reaction system contains at least pyruvate oxidase, FAD, thiamine pyrophosphate, phosphate and a salt which liberates ions selected from the group consisting of calcium cobalt, magnesium and manganese.
A kit for the analysis of pyruvic acid, comprising a plurality of separate compositions one of which contains at least pyruvate oxidase, FAD, thiamine pyrophosphate and phosphate and another of which contains at least a salt which liberates an ion selected from the group consisting of calcium, cobalt, magnesium and manganese.
Dependencies are inferred from the claim text.