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AssigneeAsahi Kasei Kogyo Kabushiki Kaisha
InventorsYoshinori Takenaka, Nobuaki Tsuda, Toru Kuroda
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Lymphocytes are advantageously separated from blood or other lymphocyte-containing suspensions by a method wherein the lymphocyte-containing suspension is flowed through a second filter having a mass of fibers exhibiting an average fiber diameter of greater than 10 microns but not greater than 60 microns, thereby to entrap a substantial part of granulocytes and monocytes in the mass of fibers and to obtain a granulocyte-poor and monocyte-poor lymphocyte-containing suspension; and then, the obtained suspension is flowed through a first filter having a mass of fibers exhibiting a bulk density of 0.04 to 0.40 g/cm.sup.3 and an average fiber diameter of 5 to 20 microns, said average fiber diameter being less than that of the second filter, thereby to entrap a substantial part of lymphocytes in the mass of fibers; and finally, the lymphocytes entrapped in the mass of fibers of the first filter is collected. Alternatively, another method is employed wherein the lymphocyte-containing suspension is flowed through the first filter thereby to entrap a substantial part of leukocyte components therein; and the leukocyte components entrapped in the first filter is collected to obtain a leukocyte component-rich suspension; and finally, the leukocyte component-rich suspension is flowed through the second filter thereby to entrap a substantial part of granulocytes and monocytes in the second filter and to obtain a lymphocyte-rich suspension.
An apparatus for separating lymphocytes from blood or other lymphocyte-containing suspensions, comprising:
A method of separating lymphocytes from a lymphocyte-containing suspension comprising the steps of:
A method of separating lymphocytes from a lymphocyte-containing suspension comprising the steps of:
Dependencies are inferred from the claim text.