Radioenzymatic assay of catecholamines
AssigneeThe Upjohn Company
InventorsGarland A. Johnson, Jacob D. Peuler
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Abstract
A new radioenzymatic assay for catecholamines utilizing the catechol-O-methyl transferase transfer of a methyl group from a labeled methyl donor to the catecholamines followed by isolation of the O-methylated(.sup.3 H)catecholamine and the subsequent measurement of radioactivity.
Claims4
A method for increasing the intrinsic affinity of catechol-O-methyl transferase for compounds having the catechol nucleus which comprises incubating with catechol-O-methyl transferase a magnesium cation of oxidation number +2, glutathione, a mammalian system selected from the group consisting of the supernatant of deproteinized tissue homogenate, blood serum or plasma, and biological fluid, the methyl donor S-adenosyl-L-methionine (.sup.3 H) methyl and an enzyme activity increasing effective amount of ethylene glycol bis(aminoethylether)-N,N'tetraacetic acid, wherein the the transferase, magnesium, glutathione and methyl donor are present in quantities which permit substantially all the catechol nuclei to be O-methylated.
Dependencies are inferred from the claim text.