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OwnerAnnette R. Saint-Firmininventor
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The process of embryogenesis in vitro whereby single embryonic cells are obtained and from which normal, healthy plants can be produced. The process is capable of making, in vitro, any metabolite producible by the whole plant, for example tropane alkaloids. This process of embryogenesis in vitro comprises the steps of inducing from any plant material, for example, from plant material of the families Solanaceae and Erythroxylaceae, the formation of a partially dedifferentiated tissue comprised of mitotic cells, such as callus and tumor cells, preparing a cell culture therefrom, rejuvenating the cells to return them to the embryonic state through controlled environmental conditions, for example, in the case of Solanaceae and Erythroxylaceae through temperature and light, differentiating the embryonic cells to restore the ageing process thereof whereby the tissue function, as part of their normal growth pattern, will give embryos and consequently metabolites. At this point qualitative and quantitative changes in the pattern of the metabolites can be induced. The process of producing a non-cyto-toxic-metabolite, from plant material. The process of obtaining mixed fermentation of cultured cells from higher plants whereby a differentiated cell producing the messenger undergoes embryogenesis. The process of inducing symbiotic relationship between cultured cells of higher plants and microorganisms for producing physiologically active compounds as for example anti-neoplastic agents. The process of inducing tumor in plant by stimulating the endogenic hormone of the cells with an exogenic supply. The novel products obtained by the foregoing processes.
A process for inducing tumor in vitro comprising treating a growing point of a seedling with a first hormone exhibiting auxin activity and thereafter treating the same growing point of the seedling with a second different hormone exhibiting auxin activity.
The process of embryogenesis in vitro comprising inducing tumor formation in a plant to produce mitotic cells, rejuvenating the mitotic cells in vitro to produce embryonic cells by means of a controlled environment wherein the temperature range is from about 12.degree. to 27.degree. C., the light energy level is from about 2,000 to 400,000 ergs per sq. cm. per second, the spectral range is up to about 730 nm, for a selected photoperiod, and in a suitable nutrient medium, differentiating the embryonic cells to obtain therefrom embryos.
The process of producing metabolites and inducing metabolite pattern changes in a free cell culture comprising establishing a free cell culture of mitotic cells, rejuvenating and differentiating the cells under a controlled environment as set forth in claim 6 and modifying one or more of the factors of the preselected environment.
The process of producing a preselected metabolite comprising the steps of rejuvenating in vitro mitotic cells of a plant producing the preselected metabolite to produce embryonic cells by means of a controlled environment as set forth in claim 6, differentiating the embryonic cells, obtaining embryos therefrom, and extracting the preselected metabolite from said embryos.
The process of claim 16, further comprising the extraction of at least one steroidal compound or cinnamic derivative.
A process of differentiating undifferentiated plant cells comprising culturing the plant cells to be differentiated with embryonic cells obtained from plant material of one of the species selected from Hyoscyamus and Duboisia.
A process of differentiating undifferentiated plant cells comprising mixing the plant cells to be differentiated with a cinnamic derivative derived from plant material of one of the species selected from Hyoscyamus and Duboisia.
A process of differentiating undifferentiated plant cells comprising culturing plant cells to be differentiated with differentiated plant cells from another plant.
The process of inducing differentiation in undifferentiated Catharanthus sp. cells comprising culturing said cells with differentiated cells from plant material of the Solanaceae of Erythroxylaceae families.
The process of inducing differentiation in undifferentiated Papaver sp. cells comprising cultivating differentiated cells from plant material of the Solanaceae or Erythroxylaceae families with said cells to be differentiated.
A process of producing metabolites comprising culturing fungi imperfecti with plant material from the family Celastraceae, said plant material being selected from the group consisting of embryos, mitotic cells, differentiated cells, and explants.
Dependencies are inferred from the claim text.