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US 4,241,181 AGranted
Broth medium for detection of DNAse-positive microorganisms
AssigneeMinnesota Mining and Manufacturing Company
InventorMarlys E. Lund
Life of this patent
Estimating the term…
Abstract
A broth medium for the detection of DNAse-positive microorganisms. The medium contains conventional nutrients for growing the microorganisms, a source of essential divalent cations to insure activity of the DNAse and a biological indicator comprising DNA, toluidine blue and lambda carrageenan in sufficient quantity so that the medium turns from blue to a visually distinguishable reddish-pink or reddish-violet color in the presence of DNAse-positive microorganisms.
Claims11
A broth medium for the detection of DNAse-positive microorganisms comprising:
- (a) a source of conventional nutrients to support growth of said microorganisms;
- (b) a source of essential divalent cations in sufficient concentration to insure activity of said DNAse;
- and
- (c) a biological indicator comprising DNA, toluidine blue and lambda carrageenan in sufficient quantity so that said medium turns to a visually distinguishable reddish-pink or reddish-violet color in the presence of DNAse-positive microorganisms.
1 more element
A broth medium for DNAse-positive microorganisms comprising about:
- 0.1 gram glucose
- 0.2 gram peptone
- 0.1 gram beef extract
- 0.25 gram K.sub.2 HPO.sub.4
- 0.01 gram lambda carrageenan
- 0.0225 gram DNA
- 0.15 ml 2% (W/V) toluidine blue 0
- 0.25 ml 30% (W/V) MgSO.sub.4.7H.sub.2 O
- 0.05 ml 30% (W/V) CaCl.sub.2.2H.sub.2 O
- 0.25 ml 10X yeast nitrogen base
- 100 ml Distilled water.
8 more elements
Dependencies are inferred from the claim text.