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AssigneeEastman Kodak Company
InventorsTheodore W. Esders, Charles T. Goodhue
Estimating the term…
A method is described for the assay of glycerol (as either free glycerol or a fatty acid ester of glycerol) in aqueous liquids such as blood serum. The method comprises the steps of I: contacting in the presence of an electron acceptor (a) a sample to be assayed and (b) a novel reagent composition comprising 1. optionally, a lipase which hydrolyzes triglycerides to glycerol; 2. glycerol kinase; 3. adenosine triphosphate; 4. .alpha.-glycerophosphate oxidase to produce a detectable change in the presence of triglyceride or a general positive sample; and II: detecting the occurrence of said detectable change. The lipase is included when fatty acid esters of glycerol (i.e., triglycerides) are to be detected. Free glycerol from whatever source can be detected with a composition comprising 2-4 above. According to a preferred embodiment, the electron acceptor is oxygen and the reagent composition also includes a hydrogen peroxide indicator composition, i.e., reagents which interact with hydrogen peroxide to produce a detectable product. The method can be used for assay of ATP. The method and reagent composition for performing the disclosed assays can be incorporated into a matrix of absorbent material.
A method for quantifying triglycerides in an aqueous liquid comprising the steps of:
A method for the quantification of triglycerides in an aqueous liquid comprising the steps of:
A method for the quantification of triglycerides in an aqueous liquid comprising the steps of:
A method for the assay of triglycerides in an aqueous liquid comprising the steps of:
A method for the assay of triglycerides in an aqueous liquid comprising the steps of:
A method for quantifying triglycerides in blood serum comprising the steps of:
A composition for the quantification of triglycerides in aqueous liquid, the composition comprising:
A composition for the quantification of triglycerides in aqueous liquid comprising:
A composition for the quantification of triglycerides in aqueous liquid comprising:
A method for quantifying glycerol in aqueous liquid comprising the steps of:
A method for quantifying glycerol in aqueous liquids comprising the steps of:
A composition for the quantification of glycerol in an aqueous liquid comprising:
A composition for the quantification of glycerol in an aqueous liquid comprising:
A composition for the quantification of glycerol in an aqueous liquid comprising:
A composition for the quantification of adenosine triphosphate in aqueous solution comprising:
An element for detecting triglycerides comprising an absorbent material which contains therein:
An element for detecting triglycerides comprising an absorbent material which contains therein:
An element for detecting glycerol comprising an absorbent material which contains therein:
An element for detecting adenosine triphosphate comprising an absorbent material which contains therein:
An element for detecting adenosine triphosphate comprising an absorbent material which contains therein:
A composition for quantification of glycerol kinase in an aqueous liquid comprising:
A composition for quantification of lipase in an aqueous liquid comprising:
A composition for quantification of .alpha.-glycerophosphate in an aqueous liquid comprising:
An element for detecting glycerol kinase in an aqueous liquid comprising an absorbent material which contains therein:
An element for detecting lipase in an aqueous liquid comprising an absorbent material which contains therein:
Dependencies are inferred from the claim text.