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AssigneeEastman Kodak Company
InventorsPaul B. Gilman, Jr., Robert T. Belly, Thaddeus D. Koszelak, Seymour Zigman
Estimating the term…
Methine dyes which inhibit cell growth are identified by (1) determining the reduction potential of the dye, (2) determining the ability of the dye to sorb to the cells and (3) selecting those dyes which have a reduction potential more negative than about -0.8 volt and which are sorbed to the cells.
A process for selecting methine dyes which inhibit cell growth, the process comprising (1) determining the reduction potential (E.sub.R) of the dyes, (2) determining the ability of the dyes to bind to the cells and (3) selecting those dyes which have a reduction potential more negative than about 31 0.8 volt and which bind to the cells to the extent of 10% or greater.
A process for selecting antimicrobial methine dyes, the process comprising (1) determining the reduction potential (E.sub.R) of the dyes, (2) determining the ability of the dyes to bind to the cells of the microbes and (3) selecting an antimicrobial agents those dyes which have a reduction potential more negative than about -0.8 volt and which bind to the microbial cells to the extent of 10% or greater.
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The polymethine chain containing an odd number of >CH- or >C[alkyl]- groups: one >CH- group, e.g. cyanines, isocyanines, pseudocyanines
Five-membered rings: condensed with carbocyclic rings
Six-membered rings: condensed with carbocyclic rings
Having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms: five-membered rings with one nitrogen atom and either one oxygen…
Term ended 25 Sep 1998 (estimated)
USPTO file history isn’t available for this patent, so only indexed dates are shown.
This invention relates to a process for selecting methine dyes which will inhibit cell growth.
Methine dyes (also referred to as methylidyne dyes) comprise a methine chain (i.e., a chain of carbon atoms with alternating double and single bonds) terminated at each end with a hetero atom. The terminal hetero atoms are typically nitrogen or oxygen atoms in various combinations, and generally they are contained in or attached to an unsaturated cyclic nucleus. Typical methine dyes are cyanine dyes, merocyanine dyes and oxonol dyes.
These dyes have found wide use in photography and related arts where they are employed, inter alia, as spectral sensitizers. The dyes are used to extend the spectral response of silver halide and other photosensitive materials to regions of the spectrum where they do not have inherent or native sensitivity.
A. Preparation of Bacillus pumilus, strain K-1 culture in nutrient broth.
Nutrient broth was prepared by mixing 8 grams of BACTO dehydrated nutrient broth per liter of distilled water. (BACTO products are sold by the Difco Company.) The nutrient broth was sterilized in an autoclave for 20 minutes at 121.degree. C. and 20 psi. Samples of the nutrient broth were then inoculated with Bacillus pumilus strain K-1 culture. The inoculated samples were then incubated at 37.degree. C. for forty-eight hours.
B. Preparation of nutrient agar plates: