Theophylline antigens and antibodies
AssigneeSyva Company
InventorsPrithipal Singh, Mae W. Hu
Life of this patent
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Abstract
Theophylline derivatives are provided for preparing reagents for use in competitive protein binding assays and for use as reagents in competitive protein binding assays. Theophylline is substituted at the 3 position and conjugated to antigens for production of antibodies which specifically recognize theophylline as distinct from structurally similar analogs such as caffeine. Enzyme conjugates are provided which find use for measuring the amount of theophylline in a sample suspected of containing theophylline. A method is provided employing the reagents for the determination of theophylline.
Claims18
A compound of the formula ##STR5## wherein:
- PAA is a poly(amino acid) of at least about 5,000 molecular weight;
- n is at least 1 and up to the molecular weight of PAA divided by 1,500;
- and
- R is a linking group of from 1 to 12 atoms other than hydrogen which are carbon, oxygen, nitrogen and sulfur.
A compound of the formula ##STR7## wherein:
- PAA is an antigenic poly(amino acid) of from about 10,000 to 600,000 molecular weight and n is in the range of from about 4 to 250.
A compound of the formula ##STR8## wherein:
- ENZ is an enzyme;
- n.sup.1 is an integer which on the average is in the range of 1 to the molecular weight of the enzyme divided by about 2,000;
- R.sup.2 is a bond or linking group;
- X and X.sup.1 are the same or different and are oxygen, imino or sulfur;
- m and p are integers of from 0 to 1 and wherein at least one of m or P is 1.
A compound of the formula ##STR9## wherein:
- ENZ is an enzyme;
- and
- n.sup.1 is on the average in the range of 2 to 16.
Dependencies are inferred from the claim text.