Calibrator composition based upon dialyzed blood serum
AssigneeEastman Kodak Company
InventorDoyle E. Hill
Life of this patent
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Abstract
A calibrator composition is disclosed in which a bias due to a dialyzed blood serum matrix is reduced to an acceptable level by the addition of a compatible, organic material. Methods of preparing and using the same are also disclosed.
Claims9
In a calibrator composition for use in calibrating an analytical system in which an aqueous albumin-containing liquid is assayed for one or more non-proteinaceous analytes contained therein by an indicator that binds to albumin having free sites, said composition comprising, in admixture
- a pre-selected amount of such analytes and
- a blood serum matrix comprising albumin having free-binding sites;
- the improvement wherein said composition contains a compatible, organic, albumin site-binding material in an amount sufficient to reduce the bias arising from the presence of said free-binding sites.
In a reduced-bias calibrator composition for calibrating an analytical system for the assay of non-proteinaceous analytes by an indicator that binds to albumin having free sites, the composition comprising, in admixture,
- a pre-selected amount of analyte and
- a blood serum matrix comprising albumin separated from molecules at least as small as those having a molecular weight of about 1,000, said albumin having free dye-binding sites formed by said separation;
- the improvement wherein said composition contains a compatible, organic material capable of occupying said sites, in an amount sufficient to reduce the bias arising from the presence of free-binding sites.
In a calibrator for calibrating an analytical system for the assay of a non-proteinaceous analyte by an indicator that binds to albumin having free sites, said calibrator comprising, as a composition,
- a pre-selected amount of the analyte and
- a dialyzed blood serum matrix comprising albumin having free-binding sites;
- the improvement wherein said calibrator contains a compatible, organic albumin site-binding material in an amount sufficient to reduce the bias arising from the presence of free-binding sites.
A method of preparing a reduced bias calibrator composition for calibrating an analytical system for the assay of non-proteinaceous analytes by an indicator that binds to albumin having free sites, comprising the steps of
- (a) dialyzing a blood serum matrix to separate albumin from molecules at least as small as those having a molecular weight of about 1,000;
- and
- (b) adding to said albumin, in either order, a pre-selected amount of a non-proteinaceous analyte and a compatible organic, albumin site-binding material in an amount sufficient to reduce the bias of the composition arising from the presence of free-binding sites.
A method of calibrating an analytical system for the assay of a predetermined analyte, using a substrate capable of generating a dye in response to the analyte, said dye being capable of binding with albumin having free sites, the method comprising the steps of
- (a) adding to successive test compositions at least two samples of a calibrator composition comprising at least two different pre-selected amounts of said analyte, a blood serum matrix including albumin having free-binding sites, and an amount of a compatible, organic albumin site-binding material sufficient to bind to said free-binding sites and to reduce the bias arising from the presence of free-binding sites;
- (b) measuring the density of said dye in the test composition;
- and
- (c) thereafter attributing the density measured to the pre-selected analyte amounts, wherein a calibration curve can be established for density measurements other than those detected in said measuring step.
A calibrator composition for use in calibrating an analytical system in which an aqueous liquid is assayed for one or more non-proteinaceous analytes contained therein by an indicator that binds to albumin having free sites, said composition comprising, in addition to said analytes,
- (a) a blood serum matrix comprising albumin having free-binding sites;
- and
- (b) a compatible, organic, albumin site-binding material in an amount sufficient to reduce the bias arising from the presence of said free-binding sites.
Dependencies are inferred from the claim text.