Element and assay for albumin
AssigneeEastman Kodak Company
InventorTai-Wing Wu
Life of this patent
Estimating the term…
Abstract
Analytical elements and methods for the detection of albumin in liquid samples feature an albumin-active complex comprising a detectable species bound to a carrier. The detectable species has a sufficiently high affinity for albumin that, when contacted with a liquid solution containing albumin, the detectable species separates from the albumin-active complex and becomes bound to albumin to form a complex comprising albumin and detectable species. The presence of albumin can be detected and, if desired, quantified by determining either the reduction of detectable species in the albumin-active complex of the formation of a complex comprising albumin and detectable species.
Claims13
An analytical element for the detection of albumin in a liquid sample,
- said element comprising a reagent zone which contains an albumin-active complex comprising a detectable species bound to a carrier;
- said detectable species having a sufficiently high affinity for albumin that, when contacted with a liquid solution containing albumin, said detectable species separates from said albumin-active complex and becomes bound to albumin to form a complex comprising albumin and detectable species;
- whereby albumin can be detected by (a) the presence of detectable species bound to albumin, or (b) a reduction in the amount of detectable species in said albumin-active complex.
An analytical element for quantifying the amount of albumin in a liquid sample,
- said element comprising an initially dry reagent zone which contains an albumin-active complex comprising a detectable species bound to a carrier;
- said detectable species having a sufficiently high affinity for albumin that, when contacted with a liquid solution containing albumin, said detectable species selectively separates from said albumin-active complex and becomes bound to albumin to form a complex comprising albumin and detectable species;
- wherein the amount of albumin present in said liquid sample can be quantified by determining the amount of detectable species which separates from said albumin-active complex and becomes bound to albumin.
An analytical element for quantifying the amount of albumin in a liquid sample,
- said element comprising a radiation-transmissive support having thereon an isotropically porous, opaque spreading layer and a reagent layer between said support and said spreading layer, said reagent layer being in fluid contact with said spreading layer under conditions of use;
- said reagent layer comprising a colored or fluorescent albumin-active complex comprising a detectable species bound to a carrier;
- said detectable species having a sufficiently high affinity for albumin that, when in fluid contact with a liquid solution containing albumin, said detectable species selectively separates from said albumin-active complex and becomes bound to albumin;
- whereby the amount of albumin present in said liquid sample can be quantified by determining the reduction in color or fluorescence of said albumin-active complex.
In a method for the detection of albumin in a liquid sample,
- said method comprising contacting said liquid sample with a detectable species, forming a complex between said detectable species and any albumin present in said liquid sample, and detecting, directly or indirectly, the formation of said complex;
- the improvement wherein:
- said detectable species which is contacted with said liquid sample is in the form of an albumin-active complex comprising said detectable species and a carrier;
- and,
- said detectable species has a sufficiently high affinity for albumin that, when said albumin-active complex is contacted with a liquid sample comprising albumin, said detectable species separates from said carrier and forms a complex with albumin.
In a method for measuring the amount of albumin in a liquid sample,
- said method comprising contacting said liquid sample with a detectable species, forming a complex between said detectable species and albumin, and quantifying the amount of albumin in the sample by detecting, directly or indirectly, the amount of said complex formed;
- the improvement wherein:
- said detectable species which is contacted with said liquid is in the form of an albumin-active complex comprising said detectable species and a carrier;
- and,
- said detectable species has a sufficiently high affinity for albumin that, when said albumin-active complex is contacted with a liquid sample comprising albumin, said detectable species separates from said carrier and forms a complex with albumin.
A method for quantifying the amount of albumin in a liquid sample, said method comprising:
- (a) placing in fluid contact:
- (i) said liquid sample, and
- (ii) an albumin-active complex comprising a detectable species bound to a carrier;
- said detectable species having a sufficiently high affinity for albumin that, when contacted with a liquid solution containing albumin, said detectable species separates from said albumin-active complex and becomes bound to albumin, the amount of detectable species which separates from said albumin-active complex being indicative of the amount of albumin present in said liquid sample;
- whereby said detectable species selectively separates from said carrier and becomes bound to albumin in said liquid sample;
- and
- (b) selectively detecting the amount of detectable species separated from said albumin-active complex.
A method for quantifying the amount of albumin in a liquid sample, said method comprising:
- (a) contacting said liquid sample with an albumin-active complex comprising a detectable species bound to a carrier;
- (b) separating said detectable species from the carrier of said albumin-active complex, and binding said detectable species of albumin;
- and
- (c) determining the amount of detectable species which separates from said albumin-active complex and becomes bound to albumin.
Dependencies are inferred from the claim text.