Process for culturing methanol-utilizing yeasts
AssigneesAgency of Industrial Science and Technology, Ministry of International Trade & Industry, Mitsubishi Gas Chemical Company, Inc.
InventorsKenzo Tonomura, Teizi Urakami
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Abstract
A process for culturing methanol-utilizing yeasts, wherein a methanol-utilizing yeast is cultured in a medium containing methanol as a main carbon source, using as seeds yeast cells obtained by culturing the yeast in a culture liquor containing methanol at a concentration higher than 0.1 wt. % and not more than 6 wt. %.
Claims10
A process for shortening the growth induction period of methanol-utilizing yeasts which comprises preculturing a yeast capable of utilizing methanol in a seed culture medium containing methanol as a main carbon source in a concentration higher than 0.1 weight % and not more than 6 weight % and additionally containing nutrient nitrogen sources and inorganic compounds to obtain a seed yeast, inoculating the seed yeast into an aqueous nutrient fermentation medium containing methanol as a main carbon source, the methanol concentration in the seed culture liquor being within said range of higher than 0.1 weight % and not more than 6 weight % at the time of inoculation thereof into the fermentation medium, and cultivating the yeast in the fermentation medium in a continuous manner.
A process for shortening the growth induction period of the microorganism Candida alcomigas NRRL Y-8023 which comprises pre-culturing said microorganism in a seed culture medium containing methanol as a main carbon source in a concentration higher than 0.1 weight % and not more than 6 weight % and additionally containing nutrient nitrogen sources and inorganic compounds to obtain a seed yeast, and then inoculating and cultivating said seed yeast in an aqueous nutrient fermentation medium containing methanol as a main carbon source, the methanol concentration in the seed culture liquor being within said range of higher than 0.1 weight % and not more than 6 weight % at the time of inoculation thereof into the fermentation medium.
A process for shortening the growth induction period of the microorganism Torulopsis methanofloat NRRL Y-8028 which comprises pre-culturing said microorganism in a seed culture medium containing methanol as a main carbon source in a concentration higher than 0.1 weight % and not more than 6 weight % and additionally containing nutrient nitrogen sources and inorganic compounds to obtain a seed yeast, and then inoculating and cultivating said seed yeast in an aqueous nutrient fermentation medium containing methanol as a main carbon source, the methanol concentration in the seed culture liquor being within said range of higher than 0.1 weight % and not more than 6 weight % at the time of inoculation thereof into the fermentation medium.
Dependencies are inferred from the claim text.