Preparation of trichloro-s-triazine activated supports for coupling ligands
AssigneeNew York University
InventorsLeonard T. Hodgins, Thomas H. Finlay, Alan J. Johnson
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Abstract
Proteins and non-protein affinity ligands are covalently bonded to trichloro-s-triazine activated supports. The activated support is prepared by reacting a water-free insoluble solid support with trichloro-s-triazine in a non-aqueous medium and neutralizing HCl generated during the reaction with a tertiary amine which does not form an insoluble complex with the trichloro-s-triazine.
Claims16
In a method for coupling substances selected from the group consisting of protein ligands and non-protein affinity ligands by covalent chemical bonds to insoluble solid supports for the preparation of solid phase catalysts and biospecific adsorbents comprising the steps of reacting the insoluble solid support material with trichloro-s-triazine to produce an activated support and thereafter, coupling the ligand to the activated support, the improvement wherein the production of the activated support is carried out by the steps comprising:
- (a) reacting a water-free insoluble solid support material with trichloro-s-triazine in a non-aqueous medium comprising an organic solvent which is compatible with the support material and is also a solvent for trichloro-s-triazine;
- (b) neutralizing the HCl generated during the reaction with a tertiary amine which is soluble in said organic solvent and which does not form an insoluble complex with trichloro-s-triazine in said organic solvent;
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- (c) washing the obtained dichloro-s-triazine activated support with an additional amount of said organic solvent.
Dependencies are inferred from the claim text.