Process for determining formate and reagent therefor
AssigneeBoehringer Mannheim GmbH
InventorsGerhard Michal, Rolf Laube, Albert Roder, Walter Schneider
Life of this patent
Estimating the term…
Abstract
Formate, or compounds convertible into formate, is determined by reacting same in the presence of formate dehydrogenase (FDH) and of a hydrogen acceptor, e.g., NAD, wherein the formate dehydrogenase is from Candida boidinii DSM 941, to result in stoichiometric reactions. The process can be used for the removal of formate from solutions containing same.
Claims14
Process for the determination of a formate compound selected from formate, or a compound convertible into formate, which process comprises reacting said formate compound in the presence of formate dehydrogenase from Candida boidinii DSM 941 and a hydrogen acceptor, and then measuring the NADH formed as a measure of the formate compound initially present.
Process for the removal of a formate compound selected from formate and compounds convertible into formate which process comprises reacting the composition containing formate in the presence of formate dehydrogenase from Candida boidinii DSM 941 and a hydrogen acceptor, whereby said formate is quantitatively converted.
Reagent for the determination of a formate compound selected from formate and compounds convertible into a formate, which reagent comprises formate dehydrogenase from Candida boidinii DSM 941 in an amount sufficient to react with the formate compound to be determined, and a hydrogen acceptor and a buffer in an amount sufficient to quantify the formate to be determined.
Dependencies are inferred from the claim text.