US 4,229,440 AGranted

Pharmaceutical composition containing the polysaccharide KGF-C as active ingredient

AssigneeFujiya Confectionery Company Limited

InventorsMitsugu Murofushi, Kuniaki Sasaki, Michio Shiomi, Yoshio Uchida, Tokihisa Hattori, Keniti Takeda

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Abstract

KGF-C is obtained by grinding kefir grain, adding water to the ground kefir grain and heating, homogenizing the mixture and centrifuging it, adding ethanol to the obtained supernatant to obtain a precipitate, dissolving the precipitate into water, purifying the obtained liquid and freeze-drying the remaining liquid. The obtained KGF-C is soluble in water and is composed of polysaccharide as principal component and contains a very small amount of nitrogenous component, and, as sugar composition, is composed of glucose and galactose in the ratio 1:1. This KGF-C has strong antitumor action by oral administration.

Claims1

Dependent claims

A composition effective for the treatment of sarcoma-180 and Ehrlich carcinoma tumors, comprising a physiologically acceptable excipient and an effective amount of KGF-C having the following physical and chemical properties:

(1) Appearance:
tasteless and odorless white powder having a little hygroscopicity;
(2) Solubility:
soluble in water and pyridine and insoluble in alcohol, glacial acetic acid, acetone, benzene, carbon tetrachloride, chloroform, ether and tetrahydrofuran;
(3) The pH of 1% aqueous solution is 6.89;
(4) Sugar qualitative reaction:
with respect to the 1% aqueous solution and liquid hydrolyzate thereof, Molish tests are positive in both 1% aqueous solution and 0.1% liquid hydrolyzate and Tollens reaction test is negative in 1% aqueous solution but is positive in 0.1% liquid hydrolyzate;
(5) Specific optical rotation:
(.alpha.).sub.D.sup.20 =+62.0.degree. C. (C=1, water);
(6) Sugar content:
99% is shown by phenol sulfuric acid method;
KGF-C is composed of polysaccharide as principal component, and a very small amount of nitrogenous component;
(7) Sugar composition:
when KGF-C is hydrolyzed by 0.5% H.sub.2 SO.sub.4 at 110.degree. C. for 5 hours and the sugar composition is determined by thin layer chromatography and gas chromatography, the results show that it is composed of glucose and galactose in the ratio of 1:1;
(8) Ultraviolet absorption spectrum:
no particular absorption is observed;
(9) Infrared absorption spectrum:
the spectrum is shown in the drawing;
and
(10) Elemental analysis:
C:
41.83%, H:
6.19%, N:
0.20%, S:
below 0.2% and P:
below 0.2%.

23 more elements

Dependencies are inferred from the claim text.