Preparation of blood fraction
OwnerBirger E. G. Blomback, Dagny B. Hesselinventors
Life of this patent
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Abstract
Concentrates of one of the coagulation factors II, V and VIII or a mixture thereof or blood plasma free from one or more of the coagulation factors VII, IX, X, XI and XII can be prepared by adding a reducing substance comprised of dithiothreitol, dithioerythritol, lipoic acid, mercaptoethanol, or a borohydride to blood or plasma, dithiothreitol being preferred. The amount of reducing substance and the period of time for interaction are so adapted to obtain the product desired, whereafter the blood and plasma, respectively, are optionally fractionated in a known manner.
Claims10
A process for the preparation of a blood component selected from the group consisting of a concentrate of at least one of the coagulation factors II, V and VIII and mixtures thereof and plasma free from one of the factors VII, IX, X, XI and XII and mixtures thereof, said process comprising:
- (1) adding a reducing agent selected from the group consisting of dithiothreitol, dithioerythritol, lipoic acid, mercaptoethanol and borohydrides to blood or plasma, the reducing agent added in an amount and for a reaction time sufficient to isolate the desired blood component, and
- (2) fractionating the blood and plasma, respectively, to isolate said blood component.
Dependencies are inferred from the claim text.