Vaccine for prophylaxis of trichophytosis in horse and method of preparing same

Abstract

The invention provides a vaccine for prophylaxis of trichophytosis in the horse which comprises a suspension of microconidia of the immunogenic strain Trichophyton equinum in a sterile physiological salt solution having a pH from 6.2 to 7.0, the concentration of the microconidia ranging from 30 to 45 million microconidia in 1 ml of sterile saline solution. The invention also provides a method for preparing said vaccine for prophylaxis of trichophytosis in the horse which involves growing the immunogenic culture Trichophyton equinum on a solid culture medium at a temperature of 26.degree. to 28.degree. C. for 15 to 25 days until optimum amounts of microconidia of said culture have accumulated, after which the resulting propagated biomass is separated, homogenized to prepare a suspension of separate microorganism cells, and the end product finally isolated by lyophilic drying. The use of the vaccine, prepared by the inventive method, rules out trichophytosis morbidity among horses to abolish losses due to untimely salts of pedigree and sporting horses, and also prevents infection of man. When tested on a mass scale, the vaccine proved highly efficacious (95-98%).

Dependencies are inferred from the claim text.