Process for extracting proteins from milk using silica and anion exchange resins
AssigneeRhone-Poulenc Industries
InventorBernard Mirabel
Life of this patent
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Abstract
Process for extracting proteins from milk which consists first of extracting the proteins, other than casein, by putting skimmed milk in contact with at least one anion exchanger resin and with silica, fixation of the proteins, then elution and finally separating the casein remaining in solution from the lactose and the mineral salts. This process is used in the dairy industries to prepare proteins usable in the food industry, the dietetic products industry, the pharmaceutical industry and the animal care products industry.
Claims12
Process for extracting proteins and casein from milk, characterized in that the proteins other than the casein are first extracted by putting skimmed milk into contact first with at least one anion exchanger resin and then in a subsequent and separate step with silica in the absence of an ion exchange resin, or by putting skimmed milk into contact first with silica in the absence of an ion exchange resin then in a subsequent and separate step with at least one anion exchanger resin whereby proteins become fixed on the anion exchange resin and the silica, leaving a solution containing casein mineral salts and lactose and eluting the protein and separating the casein remining in solution from the mineral salts and lactose.
The process for extracting proteins from milk comprising separating the lactose and mineral salts from skimmed milk by ultrafiltration or exclusion chromatography;
- extracting the proteins other than casein by putting the milk into contact first with at least one anion exchanger resin and then in a subsequent and separate step with silica in the absence of an ion exchange resin, or by putting the milk into contact first with silica in the absence of an ion exchange resin and then in a subsequent and separate step with at least one anion exchanger resin whereby proteins become fixed to the anion exchange resin and to the silica, and eluting the fixed protein from the anion exchange resin and the silica, leaving the native casein in solution.
The process for extracting proteins from milkserum, in which the milkserum is put into contact with first at least one anion exchanger resin and then in a subsequent and separate step with silica in the absence of an ion exchange resin, or by putting the milkserum into contact first with silica in the absence of an ion exchange resin and then in a subsequent and separate step with at least one anion exchanger resin, at a pH higher than 4, whereby proteins become fixed to the anion exchange resin and to the silica and eluting the fixed proteins from the anion exchange resin and from the silica.
Dependencies are inferred from the claim text.