Method for producing a peptidase
AssigneeAjinomoto Co., Inc.
InventorsHirofumi Hiraga, Ryuichi Miyajima, Koji Mitsugi
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Abstract
A method is provided for producing a peptidase capable of substantially completely hydrolyzing protein into its constituent amino acids which comprises culturing a strain of filamentous fungus, belonging to one of the species Aspergillus oryzae and Aspergillus soyae and characterized in that said strain is capable of producing said peptidase, in a nutrient culture medium containing at least one substrate selected from the group consisting of a fatty acid having 14, 16, 18 or 20 carbon atoms and a derivative of said fatty acid, and recovering said peptidase from said culture medium. It is particularly effective to use a combination of a sugar ester of a fatty acid and a vegetable oil as substrates in a liquid culture medium.
Claims9
A method for producing a peptidase capable of substantially completely hydrolyzing protein into its constituent amino acids which comprises culturing a strain of filamentous fungus, belonging to one of the species Aspergillus oryzae and Aspergillus sojae and characterized in that said strain is capable of producing said peptidase, in a nutrient culture medium containing
- (a) a protein selected from the group consisting of soybean protein, defatted soybean, wheat, wheat bran, wheat bran extract, and mixtures thereof, and
- (b) at least one substrate selected from the group consisting of a fatty acid having 14, 16, 18 or 20 carbon atoms and a derivative of said fatty acid,
- and recovering said peptidase from said culture medium.
Dependencies are inferred from the claim text.