Stabilization of peroxidase
AssigneeAkzona Incorporated
InventorsEdward C. Dawson, Jan D. H. Homan, Bauke K. Van Weemen
Life of this patent
Estimating the term…
Abstract
New and useful stable peroxidase compositions are disclosed which are particularly useful as reagents in enzyme immunoassay (EIA) tests. The novel peroxidase compositions contain polyvalent ions of groups 3 and 4 of the Periodic Table. These novel compositions are stable even at low concentrations of peroxidase, and are relatively invulnerable to freeze-drying. Preferably, the polyvalent ions selected are those of Al, Zn, Mg, Fe, and Cu.
Claims12
Process for the stabilization of peroxidase-containing compositions for use in enzyme immunoassay tests, consisting essentially of:
- adding one or more polyvalent metal ion(s) selected from the group consisting of Mg, Ca, Sc, Ti, V, Cr, Mn, Fe, Co, Ni, Cu, Zn, Ga, and Al to an aqueous solution consisting essentially of peroxidase present from about 1 nanogram per milliliter to about 25 micrograms per milliliter, wherein the metal ion concentration is in an amount of at least about 0.0001 M.
An aqueous composition for use in enzyme immunoassay tests, consisting essentially of:
- a peroxidase present from about 1 nanogram per milliliter to 25 micrograms per milliliter;
- and one or more polyvalent metal ions selected from the group consisting of Mg, Ca, Sc, Ti, V, Cr, Mn, Fe, Co, Ni, Cu, Zn, Ga, and Al, wherein the ion is present in an amount of at least 0.0001 M.
Dependencies are inferred from the claim text.