Method of laboratory testing in water-based culture media for zones of inhibition
AssigneeAllor Foundation
InventorDamon Swanson
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Abstract
This disclosure is concerned with providing extractions of oil-based anti-microbial materials, such as essential oil extracts and the like, that can be rendered miscible with water-based culture media with which they are otherwise non-miscible, to enable zone of inhibition testing with such culture media.
Claims3
A method of enabling zone of inhibition testing of essential oil extracts with non-miscible oil-based culture media and the like, that comprises, mixing said essential oil extracts with an alcohol that, in relatively dilute concentration, does not demonstrate substantial anti-microbial activity, to form an emulsion containing an extraction of the same materials;
- diluting the alcohol emulsion with water sufficient to bring the alcohol to said relatively dilute concentration of non-anti-microbial activity;
- saturating a sensitivity disc with a sample of the diluted emulsion after contacting the disc with a water-based culture medium;
- evaporating the alcohol saturated into the disc;
- inverting and incubating the culture medium carrying the disc;
- and observing zones of inhibition produced on the disc as a result of miscible reaction with the water-based culture medium of the alcohol-extracted originally non-miscible essential oil extracts.
Dependencies are inferred from the claim text.