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US 4,228,154 AGranted
Purification of plasma albumin by ion exchange chromatography
AssigneeArmour Pharmaceutical Company
InventorsJoseph D. Fisher, Willie M. Curry, Michael E. Hrinda
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Abstract
Purified plasma albumin is obtained by ion exchange chromatographic procedures and in the absence of process steps involving precipitation or desorption of the albumin.
Claims11
In the process for isolation of purified plasma albumin wherein plasma is processed by cold alcohol fractionation to develop an albumin-containing Cohn II+III supernatant fluid, an improvement in the procedure for further isolation of albumin from proteinaceous material in the fluid, said improvement comprising the steps of:
- (a) intimately contacting said fluid with lipoprotein extractant means;
- (b) adjusting the pH of the fluid to from about 4.5 to 4.9 and intimately contacting the pH-adjusted fluid with an anionic ion exchanger means for removing from said ph-adjusted fluid proteinaceous materials having an isoelectric point below that of albumin;
- and
- (c) adjusting the pH of said to fluid from about 5.1 to 5.5 and intimately contacting the pH-adjusted fluid with a cationic ion exchanger means for removing from said pH-adjusted fluid proteinaceous materials having an isoelectric point above that of albumin,
- whereby an albumin-containing fluid product is obtained substantially free of contaminating proteinaceous materials having an isoelectric point below or above that of albumin.
2 more elements
Dependencies are inferred from the claim text.