Method for immobilizing enzymes
AssigneeJapan Atomic Energy Research Institute
InventorsMasaru Yoshida, Minoru Kumakura, Isao Kaetsu
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Abstract
Enzymes are adsorbed on an adsorbent powder in an aqueous medium and a water-miscible monomer is added thereto, and said monomer is polymerized to form a porous gel lump through which a substrate solution can freely pass. The thus formed enzyme composition has long-lasting activity and thus the enzymes can be easily separated from the reaction system and can be used repeatedly.
Claims9
A method for immobilizing enzymes or enzyme-containing cells comprising (a) adsorbing said enzymes or enzyme-containing cells on an adsorbent selected from the group consisting of inorganic natural earth adsorbents, synthetic inorganic adsorbents and active carbon in an aqueous medium, (b) admixing the aqueous dispersion of said adsorbent on which enzymes or enzyme-containing cells have been adsorbed with a polymerizable monomer selected from the groups consisting of compounds represented by the general formula ##STR3## wherein R.sup.1 is a group --CH.sub.2 CH.sub.2 -- or --CH(CH.sub.3)CH.sub.2 --, R.sup.2 is hydrogen or methyl or a group represented by ##STR4## X is hydrogen or methyl, n is an integer 2, 3 or 4 and m is an integer from 2 to 20 and (c) effecting polymerization at a temperature between -20.degree. C. and -80.degree. C., to form a porous gel lump in which enzymes or enzyme-containing cells are distributed adsorbed on inorganic adsorbent, and through which a substrate solution can freely pass.
A porous three dimensional reticular structure comprising adsorbed enzyme or enzyme-containing cells whenever obtained by (a) adsorbing said enzymes or enzyme-containing cells on an adsorbent selected from the group consisting of inorganic natural earth adsorbents, synthetic inorganic adsorbents and active carbon in an aqueous medium, (b) admixing the aqueous dispersion of said adsorbent on which enzymes or enzyme-containing cells have been adsorbed with a polymerizable monomer selected from the groups consisting of compounds represented by the general formula ##STR7## wherein R.sup.1 is a group --CH.sub.2 CH.sub.2 -- or --CH(CH.sub.3)CH.sub.2 --, R.sup.2 is hydrogen or methyl or a group represented by ##STR8## X is hydrogen or methyl, n is an integer 2, 3 or 4 and m is an integer from 2 to 20 and (c) effecting polymerization at a temperature between -20.degree. C. and -80.degree. C., to form a porous gel lump in which enzymes or enzyme-containing cells are distributed adsorbed on inorganic adsorbent, and through which a substrate solution can freely pass.
Dependencies are inferred from the claim text.