Enzymatic diagnostic composition
AssigneeW. R. Grace & Co.
InventorMurray C. Fusee
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Abstract
Disclosed herein is a method for binding enzymes comprising reacting an amino acid and/or a protein with an excess of a urethane prepolymer, curing the resulting product and coupling an enzyme thereto by use of a carbodiimide. The product of the process is also described as well as the use thereof in analyzing blood fluids. The invention is particularly adapted to binding hexokinase and glucose-6-phosphate dehydrogenase to determine creatine phosphokinase (CPK) in human blood serum.
Claims33
A diagnostic composition comprising a mixture of hexokinase and glucose-6-phosphate dehydrogenase bound in biologically-active form to a carboxylated poly(urea-urethane) polymer matrix having oxyalkylene backbone segments wherein at least 60 mole percent of the oxyalkylene units are oxyethylene said carboxylated polymer prepared by reacting an excess of a polyoxyalkylene urethane prepolymer with a monomeric amino acid, and wherein said enzymes are bonded to at least one exterior surface of said polymer matrix.
A method for analyzing human body fluids comprising contacting a first aqueous solution of said body fluids with a mixture of hexokinase and glucose-6-phosphate dehydrogenase bound in biologically-active form to a carboxylated polyurethane polymer matrix, allowing said immobilized enzymes to catalyze reaction between the components of the first aqueous solution to form a second aqueous solution and determining the amount of at least one component of said second aqueous solution, said polymer comprising the reaction product of an excess of a polyoxyalkylene urethane prepolymer and a monomeric amino acid.
A method for binding enzymes comprising reacting a monomeric amino acid with an excess of a polyoxyalkylene urethane prepolymer to form a carboxylated prepolymer, curing the carboxylated prepolymer and coupling an enzyme thereto by use of a carbodiimide.
Dependencies are inferred from the claim text.