Formalinized allergen-containing substances and production thereof
OwnerDavid G. Marshinventor
Life of this patent
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Abstract
This invention relates to the production of formaldehyde treated allergens suitable for the immunotherapy (desensitization) of individuals suffering from allergies of the immediate type. These formalinized allergen-containing materials are also useful to induce formation of antibody in mammals which cross reacts with the native allergen-containing materials.
Claims37
A process for producing a formalinized allergen of low allergenic activity in allergic humans and which is capable of inducing in mammals the formation of blocking antibody against the native allergen in significant concentration, comprising:
- (1) extracting an allergen-containing material with an aqueous solution;
- (2) removing essentially all of the low molecular weight non-allergenic substances from the soluble part of the said extract to leave the allergen-containing materials in said solution;
- (3) adding formaldehyde to the said solution of allergen-containing materials to give a concentration of formaldehyde from about 0.06 M to 5.0 M;
- (4) treating the aqueous extract of material containing said allergens with said formaldehyde solution in a non-phenolic environment at a temperature from about the freezing point of the incubation mixture up to about 40.degree. C.;
- and (5) incubating the same at a temperature below that which the allergen undergoes substantial heat denaturation and generally from about the freezing point of the incubation mixture up to about 40.degree. C. until the substantial formation of inter- or intramolecular methylene bridge linkages occurs in said solution and the allergenic determinant groups are modified to result in substantial reduction of allergenic properties while largely retaining the desired immunizing properties.
A process for producing a formalinized allergen of low allergenic activity in allergic humans and which is capable of inducing in mammals the formation of blocking antibody against the native allergen in significant concentration, comprising:
- (1) extracting an allergen-containing material with an aqueous solution;
- (2) removing essentially all of the low molecular weight non-allergenic substances from the soluble part of the said extract to leave the allergen-containing materials in said solution;
- (3) adding formaldehyde to the said solution of allergen-containing materials to give a concentration of formaldehyde from about 0.06 M to 4.0 M;
- (4) treating the aqueous extract of material containing said native allergen with said formaldehyde solution in a non-phenolic environment at a temperature from about 15.degree. C. to about 32.degree. C.;
- and (5) incubating the same at a temperature below that which the allergen undergoes substantial heat denaturation and generally from about 15.degree. C. to about 32.degree. C. until the substantial formation of inter- or intramolecular methylene bridge linkages occurs in said solution and the allergenic determinant groups are modified to result in substantial reduction of allergenic properties while largely retaining the desired immunizing properties.
A formalinized allergen of low allergenic activity in allergic humans and which is capable of inducing in mammals the formation of blocking antibody against the native allergen in significant concentration, prepared by:
- (1) extracting an allergen-containing material with an aqueous solution;
- (2) removing essentially all of the low molecular weight non-allergenic substances from the soluble part of the said extract to leave the allergen-containing materials in said solution;
- (3) adding formaldehyde solution to the said solution of allergen-containing materials to give a concentration of formaldehyde from about 0.06 M to 5.0 M;
- (4) treating the aqueous extract of material containing said allergens with said formaldehyde solution in a non-phenolic environment at a temperature from about the freezing point of the incubation mixture up to about 40.degree. C.;
- and (5) incubating the same at a temperature below that which the allergen undergoes substantial heat denaturation and generally from about the freezing point of the incubation mixture up to about 40.degree. C. until substantial formation of inter- or intramolecular methylene bridge linkages occur in said solution and the allegenic determinant groups are modified to result in substantial reduction of allergenic properties while largely retaining the desired immunizing properties.
A formalinized allergen of low allergenic activity in allergic humans and which is capable of inducing in mammals the formation of blocking antibody against the native allergen in significant concentration, prepared by:
- (1) extracting an allergen-containing material with an aqueous solution;
- (2) removing essentially all of the low molecular weight non-allergenic substances from the soluble part of the said extract to leave the allergen-containing materials in said solution;
- (3) adding formaldehyde solution to the said solution of allergen-containing materials to give a concentration of formaldehyde from about 0.06 M to 4.0 M (4) treating the aqueous extract of material containing said allergens with said formaldehyde solution in a non-phenolic environment at a temperature from about 15.degree. C. to about 32.degree. C.;
- and (5) incubating the same at a temperature below that which the allergen undergoes substantial heat denaturation and generally from about 15.degree. C. to about 32.degree. C. until substantial formation of inter- or intramolecular methylene bridge linkages occur in said solution and the allergenic determinant groups are modified to result in substantial reduction of allergenic properties while largely retaining the desired immunizing properties.
Dependencies are inferred from the claim text.