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AssigneeMorinaga Milk Industry Co., Ltd.
InventorsKatsuhiro Ogasa, Morio Kuboyama, Minoru Saito, Tsutomu Kudo, Yoshitsugu Harada, Akio Kawashiri, Eiji Takahashi
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Latex reagents for quantitative assay of a variety of steroid hormones or metabolites thereof which are contained in body fluid or excreted fluid of human beings comprise latex particles which are immunologically sensitized with a conjugate selected from a variety of steroid-serum albumin conjugates in which the steroid to be detected is bonded to serum albumin in a very small ratio ranging from 0.5 to 7 molecules per 1 molecule of the serum albumin used. Sensitization of latex particles with such a conjugate is performed using a limited amount dependent on the bonding ratio of steroid molecules per molecule of serum albumin in the conjugate and the particle size of the latex used. The latex reagents show very high sensitivity amounting to 0.2-0.04 nmole steroid equivalent/ml.
A method for preparation of a latex reagent containing latex particles sensitized with a steroid-serum albumin conjugate and suitable for immunochumical detection of steroid contained in human body fluid or excreted fluid, which comprises
A latex reagent comprising latex particles sensitized with steroid-serum albumin conjugate and suitable for immunochemical detection of steroid contained in human body fluid or excreted fluid, wherein said conjugate has a steroid bonding number in the range 0.5-7.0 molecules per molecule of serum albumin, and said latex particles are sensitized with an effective amount of said conjugate to avoid non-specific agglutination but to cause agglutination due to antigen-antibody reaction between said latex reagent and the antibody for which the steroid to be detected may act as a hapten.
In a method for the immunochemical detection of a steroid compound and/or metabolites thereof present in human body fluid or excreted fluid by forming a conjugate of a steroid and a protein material, forming sensitized latex particles by absorbing said conjugate on latex particles, obtaining an antiserum or antibody by injecting an animal with said conjugate and thereafter separating said antiserum or antibody from the blood of said animal and determining the presence of said steroid compound in a sample of body fluid by an agglutination reaction in the presence of said antiserum or antibody and said sensitized latex particles, the improvement comprising:
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