Adsorbent for urokinase and process for the production of urokinase
AssigneeNippon Soda Co., Ltd.
InventorsHiroaki Nakamura, Izumi Kumita, Yoshiji Sugita, Hiedo Takagi
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Abstract
Highly pure urokinase can be produced by affinity chromatography on the adsorbent. The adsorbent comprises water insoluble carrier to which the group of the general formula wherein n is an integer of 5 to 12, m is zero or one, and R is (m- or p-) guanidino or (m- or p-) amidino group; is bound.
Claims6
A process for the production of highly pure urokinase which comprises passing an impure aqueous solution of urokinase through a first column packed with a protein absorbent comprising a water insoluble carrier to which is bound a material selected from the group consisting of ##STR6## where l is an integer of from 3 to 10, and X is a material selected from the group consisting of hydrogen, alkyl, halogen and alkoxycarbonyl;
- and, passing the effluent therefrom through a second column packed with an adsorbent from urokinase comprising a water insoluble carrier to which is bound a material selected from ghe group of the general formula ##STR7## where n is an integer of from 5 to 12,
- m is 0 or 1, and
- R is (m- or p-) guanidino or (m- or p-) amidino group, and, eluting the adsorbed urokinase.
Dependencies are inferred from the claim text.