Method for determination of free hormones in biologic fluids
AssigneeGruppo Lepetit S.p.A.
InventorsPier B. Romelli, Francesco Pennisi
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Abstract
A method for direct determination of the free fraction of an hormone in a biological fluid containing bound and unbound hormone in an equilibrium state. A fluid sample is contacted with a solid matrix capable of reversibly binding the free hormone without sensibly affecting the equilibrium state and the amount of adsorbed hormone is determined and directly correlated with the free hormone in the biological fluid. The method is particularly suitable for direct determination of free thyroxine and triiodothyronine in serum.
Claims11
A process for determining the concentration of the free fraction of a hormone in a biological fluid containing bound and unbound hormone in an equilibrium state which comprises contacting a sample of the biological fluid with a solid matrix capable of reversibly selectively adsorbing the free hormone, whereby the equilibrium between the bound and unbound fraction of the hormone remains substantially undisturbed and determining the amount of hormone bound to said solid matrix directly correlating the adsorbed hormone with the concentration of the free hormone present in said body fluid.
Dependencies are inferred from the claim text.