Aloctin A
AssigneeAloace Company Limited
InventorIkuo Suzuki
Life of this patent
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Abstract
A glycoprotein isolated from Aloe, a plant belonging to Liliaceae, having a molecular weight of 1.8.times.10.sup.4 and a ratio of protein to sugar of 8 to 2 by weight; yielding only a single band upon SDS-polyacrylamide gel electrophoresis without 2-mercaptoethanol treatment and yielding two discrete bands upon SDS-polyacrylamide gel electrophoresis with 2-mercaptoethanol treatment; having hemagglutinating and cytoagglutinating activity for transformed cells; having mitogenic activity for lymphocytes, cap forming activity for lymphocytes and cultured transformed cells using fluorescence labeled glycoprotein; having binding reactivity with some types of serum proteins; and having the capability of activating complement components.
Claims1
A glycoprotein isolated from Aloe, a plant belonging to Liliaceae,
- (1) having a molecular weight of 1.8.times.10.sup.4 and a ratio of protein to sugar of 8 to 2 by weight;
- (2) yielding only a single band upon SDS-polyacrylamide gel electrophoresis without 2-mercaptoethanol treatment and yielding two discrete bands upon SDS-polyacrylamide gel electrophoresis with 2-mercaptoethanol treatment;
- (3) having hemagglutinating and cytoagglutinating activity for transformed cells;
- (4) having mitogenic activity for lymphocytes, cap forming activity for lymphocytes and cultured transformed cells using fluorescence labeled glycoprotein;
- (5) having binding reactivity with some types of serum proteins;
- (6) having the capability of activating serum complement components;
- (7) having an infrared spectrum whose absorption peaks appear at 1200-1240 cm.sup.-1, 1500-1520 cm.sup.-1, 1620-1640 cm.sup.-1 and 3200-3260 cm.sup.-1 ;
- and
- (8) having a nuclear magnetic resonance spectrum whose absorption peaks appear at 15-27 ppm (multiplet), 67-69 ppm (singlet), 71-77 ppm (multiplet), 99-100 ppm (singlet) and 169-180 ppm (multiplet).
Dependencies are inferred from the claim text.