Chemiluminescent naphthalene-1,2-dicarboxylic acid hydrazide-labeled polypeptides and proteins
AssigneeMiles Laboratories, Inc.
InventorsRobert T. Buckler, Hartmut R. Schroeder
Life of this patent
Estimating the term…
Abstract
Chemiluminescent-labeled conjugates of the formula: ##STR1## wherein R is hydrogen or straight chain alkyl containing 1-4 carbon atoms, n=2-6 and L(CO-- is a specifically bindable ligand, such as an antigenic protein or polypeptide, a hapten or an antibody, or a binding analog thereof, bound through an amide bond; and intermediates produced in the synthesis of such conjugates. The labeled conjugates are useful as reagents in specific binding assays for determining ligands or their specific binding partners in liquid media.
Claims11
In a chemiluminescent-labeled conjugate for use in specific binding assays of the general formula:
- L(CO)--(NH)labeling substance
- wherein L(CO)--is an antigenic polypeptide or protein bound to said labeling substance through an amide bond, characterized in that said --(NH)labeling substance has the formula ##STR31## wherein R is hydrogen or straight chain alkyl containing 1-4 carbon atoms and n=2-6.
Dependencies are inferred from the claim text.