Method and means for anesthetizing insects
AssigneeCarolina Biological Supply Company
InventorsRaymond O. Flagg, Richard L. Franks
Life of this patent
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Abstract
Insects, such as fruit flies, are quickly anesthetized while contained in a culture vessel of the type having a mouth closed by a flexible porous plug by immersing one end portion of an elongate slender applicator wand in a volatile liquid anesthetic and then inserting the applicator wand between the wall of the culture vessel and the porous plug without removal of the plug and without creating an opening therebetween to allow the insects to escape and positioning the end portion of the applicator wand inside the culture vessel to thereby quickly expose the insects in the culture vessel to the anesthetic.
Claims9
A method for anesthetizing insects, such as fruit flies, while the insects are contained in a culture vessel having a mouth closed by a flexible porous plug, said method being characterized by avoiding the necessity of removing the plug or transferring the insects to another vessel, and said method comprising applying a volatile liquid anesthetic to an applicator, inserting the anesthetic-containing applicator between the wall of the culture vessel and the porous plug without removal of the plug and without creating an opening therebetween to allow the insects to escape, and positioning the anesthetic-containing applicator inside the culture vessel and maintaining the applicator in the culture vessel while allowing the anesthetic to vaporize to thereby expose the insects in the culture vessel to the anesthetic.
A method for anesthetizing insects, such as fruit flies, with a predetermined amount of anesthetic while the insects are contained in a culture vessel having a mouth closed by a flexible porous plug, said method being characterized by avoiding the necessity of removing the plug or transferring the insects to another vessel, and said method comprising immersing an end portion of an elongate slender applicator wand to a predetermined depth in a volatile liquid anesthetic so as to obtain a predetermined desired amount of anesthetic on the wand, inserting the applicator wand between the wall of the culture vessel and the porous plug without removal of the plug and without creating an opening therebetween to allow the insects to escape, and pushing the applicator wand endwise until the anesthetic-containing end portion is positioned inside the culture vessel to thereby expose the insects therein to the anesthetic.
Means for anesthetizing insects, such as fruit flies, comprising a culture vessel having a mouth and a flexible porous plug positioned in the mouth for retaining insects in the culture vessel, an elongate slender absorbent wand of a sufficiently small diameter for being inserted between the wall of the culture vessel and the flexible porous plug without creating an opening therebetween to allow insects to escape, and a supply of volatile liquid anesthetic in which the end portion of the absorbent wand may be immersed and thereafter inserted into the vessel to thereby expose the insects to the anesthetic.
Dependencies are inferred from the claim text.