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US 4,224,034 AGranted
Assay of iron and iron binding protein reagents and methods
AssigneeAmerican Monitor Corporation
InventorsJerry W. Denney, Mark C. Outcalt
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AssigneeAmerican Monitor Corporation
InventorsJerry W. Denney, Mark C. Outcalt
Estimating the term…
The discovery of 9-(2-pyridyl)-acenaphtho[1,2-e]-as-triazine and its sulfonated form, having particular advantages when used as both a chelator and indicator of ferrous ions in organic or aqueous mediums; a process for the synthesis of those compounds in which hydrazine is used as both a reactant and as a solvent in production of intermediate compounds, eliminating the need of ethanol and the disadvantageous ether-extraction step; the use of N,N-dimethylformamide as a reaction solvent in the production of triazines; the use of the new triazine compounds to measure iron, transferrin, and UIBC/TIBC in biological systems; the use of dimethylsulfoxide and certain processes to eliminate the interferences commonly encountered in these assays; and the use of the triazine compounds to measure both total and/or reduced iron in a variety of matrices.
A process for detecting the presence of ferrous ions, which includes the step of adding, to the specimen being assayed, 9-(2-pyridyl)-acenaphtho[1,2-e]-as-triazine as both a chelator and indicator of ferrous ion.
In the quantitative determination of ferrous ion in biological samples, the step of adding 9-(2-pyridyl)-acenaphtho[1,2-e]-as-triazine, as both a chelator and indicator of ferrous ion.
A process for the quantitative determination of unsaturated transferrin in biological samples, which includes the step of adding, to the specimen being assayed, sulfonated 9-(2-pyridyl)-acenaphtho[1,2-e]-as-triazine as a chelator and indicator of ferrous ion content.
A process for the quantitative determination of total transferrin in biological samples, which includes the step of adding, to the specimen being assayed, sulfonated 9-(2-pyridyl)-acenaphtho[1,2-e]-as-triazine as a chelator and indicator of ferrous ion content.
A process for the quantitative or qualitative determination of ferrous ions in an organic or aqueous matrix, which includes the step of adding, to the specimen being assayed, 9-(2-pyridyl)-acenaphtho[1,2-e]-as-triazine as a chelator and indicator of said ions.
In a process for the direct serum quantitation of ferrous ion present in serum, the step of adding, to the specimen being assayed, dimethylsulfoxide to increase the liberation of iron bound to transferrin.
In the process of quantitatively determining the amount of iron in a serum sample, the procedural step of maintaining the incubation of the sample in the buffer system employed therewith for an extended period sufficient to minimize interferences encountered in the use of hemolyzed samples.
In a process for the determination of the oxidative state of iron ion present in a solution and/or the determination of the ratio of the oxidized to reduced form thereof, the step of adding, to the specimen being assayed, 9-(2-pyridyl)-acenaphtho[1,2-e]-as-triazine or its sulfonated form as an indicator of the oxidation state of the iron ions present in the solution.
Dependencies are inferred from the claim text.