Immunochemical testing using tagged reagents
AssigneeE. I. Du Pont de Nemours and Company
InventorRichard C. Ebersole
Life of this patent
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Abstract
The presence of a particular immunochemically reactive substance in a biological fluid can be determined using immune reagents tagged with particles which have characteristics capable of affecting electrical reactance.
Claims10
A method for determining the presence of an analyte in a body fluid consisting essentially of
- a. contacting a surface with said fluid, said surface being coated with receptor reagents specifically reactive with said analyte;
- b. contacting the coated surface with immune reagent comprising immune compound specifically reactive with the receptor reagents or with a complex of the analyte and receptor reagent, said immune compound being coated on reactance tags which are particles capable of affecting electrical reactance;
- c. removing unreacted immune reagent from the surface;
- and
- d. measuring the electric reactance of the surface.
An automated analytical system including a test surface having a receptor reagent bonded thereto, said receptor reagent being specifically reactive with a component of a body fluid, a station for the application of a body fluid sample to said test surface, means for the application of an immune reagent to the test area, said immune reagent comprising immune compounds specifically reactive with the receptor reagent or with a complex of the receptor reagent with the component of the body fluid, said immune compounds being coated on reactance tags comprising particles which are capable of affecting electrical reactance, means for the removal of excess immune reagent, and means for the detection of reactance tags on said test area.
Dependencies are inferred from the claim text.