Specific binding assay method and reagent means
AssigneeTechnion Research & Development Foundation Limited
InventorMichael Cais
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Abstract
A method and reagent means for the determination of small quantities of chemical compounds in man, animal and plants by a specific binding assay technique. The method involves the utilization of a labelling constituent comprising a conjugate of a labelling substance and a binding component said labelling substance being one or more metal atoms which can be easily determined. The labelling constituents are selected from various metalo organic derivatives or metal coordination complexes. The method is very specific and sensitive and can be successfully utilized for the determination of haptens selected from various groups such as alkaloids, barbiturates, steroids, cannabinoids, vitamins, aminoacids, tranquilizers, sugars, penicillins, etc.
Claims35
A heterogeneous specific binding assay method for assaying a liquid medium for a ligand, which method comprises:
- (a) contacting said liquid medium with reagent means which includes a metal (metallo-hapten, metallo-binder) labelled constituent comprising a conjugate of a metal labelling substance and a binding component and which forms, with said ligand to be determined, a binding reaction system producing a metal-containing bound-phase and a metal-containing free-phase of said metal labelled constituent, the quantity of metal labelling substance resulting in said metal-containing bound-phase and metal-containing free-phase being a function of the amount of said ligand present in said liquid medium.
- (b) separating said metal-containing bound-phase from said metal-containing free-phase;
- and
- (c) measuring the metal content in either said bound-phase or said free-phase.
Reagent means for use in assaying a liquid medium for a ligand, which reagent means includes a metal labelled constituent comprising a conjugate of a metal labelling substance and a binding component and which forms, in combination with said ligand, a specific binding reaction system producing a metal-containing bound-phase and a metal-containing free-phase of said labelled constituent, the quantity of labelling metal resulting in said metal-containing bound-phase being a function of the amount of said ligand present in said liquid medium, characterized in that said labelling substance is at least one metal atom which can be exactly measured.
Dependencies are inferred from the claim text.