Hydrolytic enzyme material
AssigneeGerold K. V. Klein
InventorsGerold K. V. Klein, John C. Houck
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Abstract
Disclosure relates to novel hydrolytic enzyme product derived from the pineapple plant useful for the digestion, dissection and separation of non-viable, devitalized tissue, especially eschar tissue from viable tissue in a mammalian host.
Claims8
An enzyme product obtainable from bromelain capable of debridement of devitalized tissue from a mammalian host which comprises a water soluble, heat labile protein free of caseinolytic activity having a peak isoelectric point of about 6, comprising at least two subunits, each subunit having a molecular weight of from about 14,300 to 15,000 daltons, with a characteristic absorption peak in the ultraviolet region of the spectrum at 280 nm.;
- the said product being active in the absence of sulfhydryl activation and in the presence of sulfhydryl deactivating quantities of phenylmercuric acetate, and the physiological acceptable alkali metal and acid addition salts thereof.
A pharmaceutical composition comprising a pharmaceutically acceptable excipient together with an enzyme product obtainable from bromelain capable of debridement of devitalized tissue from a mammalian host which comprises a water soluble, heat labile protein, free of caseinolytic activity, having a peak isoelectric point at about 6 comprising at least 2 subunits, each subunit having a molecular weight of from about 14,300 to 15,000 daltons with a characteristic absorbtion peak in the ultraviolet region of the spectrum at 280 nm.;
- the said enzyme being active in the absence of sulfhydryl activation and in the presence of sulfhydryl deactivating quantities of phenylmercuric acetate, and the physiologically acceptable alkali metal and acid addition salts thereof.
Dependencies are inferred from the claim text.