Method for immobilizing enzymes and microbial cells
AssigneeKansai Paint Co., Ltd.
InventorsSaburo Fukui, Tsutomu Yamamoto, Takamitsu Iida
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Abstract
A method for immobilizing enzymes or microbial cells which comprises the steps of uniformly mixing an aqueous dispersion of enzymes or microbial cells with a photo-curable resin having a number average molecular weight of 800 to 100,000, two or more photopolymerizable ethylenically unsaturated groups per molecule and hydrophilic groups, and irradiating actinic rays to the above mixture of photo-curable resin and enzymes or microbial cells. The immobilized product is advantageously used in various enzymatic reactions with stable enzyme activity.
Claims10
A method for immobilizing enzymes or microbial cells which is characterized in the steps of:
- uniformly mixing aqueous dispersion of enzymes or microbial cells with a monomer-free photo-curable resin having a number average molecular weight of 800 to 100,000, two or more photo-polymerizable ethylenically unsaturated groups per molecule and nonionic hydrophilic groups;
- and
- irradiating actinic rays having a wave length of 2500 to 6000 A to said mixture, whereby said enzymes or microbial cells are entrapped in said resin.
Dependencies are inferred from the claim text.