Liquid chromatography system with packed flow cell for improved fluorescence detection
AssigneeVarian Associates, Inc.
InventorsAhmad Abu-Shumays, Edward L. Johnson
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Abstract
In a liquid chromatography system, the effluent from the chromatographic column is passed through a detector flow cell, which is packed with a stationary phase that is adsorptive of the sample species to be detected. Detection of the sample species adsorbed at the stationary phase is effected by measuring fluorescence emitted from such species in response to electromagnetic radiation incident thereon at the flow cell. The fluorescing species, which are in equilibrium between the stationary phase and the mobile phase in the flow cell when fluorescence is being measured, can be detected at lower concentration thresholds than when the fluorescing species are in equilibrium only with the mobile phase.
Claims21
A method for enhancing the fluorescent detectability of an aflatoxin present in a solvent, said method comprising the step of passing an aflatoxin-bearing eluant from a liquid chromatography column through a flow cell packed with aflatoxin-adsorbing particles, whereby there is caused a dynamic equilibrium of the aflatoxin between the solvent of the eluant and said particles, and whereby said detectability is enhanced;
- said flow cell having a wall portion that is substantially transparent to electromagnetic radiation of a frequency that causes the aflatoxin to fluoresce.
A chromatographic method for detecting the presence of an aflatoxin in a sample material, said method comprising in sequence the steps of:
- (a) passing said sample material through a liquid chromatography column;
- (b) passing the outflow of said column through a flow cell packed with aflatoxin-adsorbing particles whereby there is caused a dynamic equilibrium of the aflatoxin between the mobile phase and said particles;
- (c) irradiating said flow cell with electromagnetic radiation of a frequency that causes the aflatoxin to fluoresce;
- and
- (d) detecting the emitted fluorescence.
A fluorescence spectrometer comprising:
- (a) a flow cell through which an aflatoxin present in a solvent and having been eluted from a liquid chromatography column can pass, said flow cell being packed with aflatoxin-adsorbing particles whereby there is caused a dynamic equilibrium of the aflatoxin between the solvent and said particles;
- (b) means for irradiating the aflatoxin with electromagnetic radiation of a frequency that causes the aflatoxin to fluoresce;
- and
- (c) means for detecting the emitted fluorescence;
- said flow cell having a wall portion that is substantially transparent to said electromagnetic radiation.
Dependencies are inferred from the claim text.