Method for the production of a vehicle substance which is capable of covalent bonding with biologically active matter
AssigneeMax-Planck-Gesellschaft zur Forderung der Wissenschafen e.V.
InventorsChristof Biebricher, Rudiger Luce
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Abstract
Process for preparing a vehicle substance capable of covalent bonding with iological material, especially enzymes, by reacting a matrix containing --OH or NH.sub.2 groups with an organic diisocyanate under anhydrous conditions. The distance between the matrix and biological material may be extended by a stepwise reaction of the matrix with an organic diisocyanate, and organic spacer containing two or more --OH and/or NH.sub.2 groups and again with an organic diisocyanate. The coupling of the vehicle material with biological material takes place in buffered aqueous solution.
Claims26
In a method for the production of a vehicle substance capable of covalent bonding with a biologically active matter which is synthetized from a vehicle containing preferably several free --NH.sub.2 or --OH groups and at least a bonding agent containing at least two reactive groups, the improvement wherein the vehicle under anhydrous conditions is reacted with an excess of organic diisocyanate in an anhydrous organic aprotic solvent in the presence of a strong basic catalyst, and the resultant vehicle substance is isolated in a storable form.
Dependencies are inferred from the claim text.