Method for the inactivation of microbial toxins and attenuation of vaccines
AssigneeSankyo Company, Limited
InventorsTakashi Okonogi, Zenpachiro Hattori, Akira Ogiso, Seiji Mitsui
Life of this patent
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Abstract
A method for the inactivation of microbial toxins and attenuation of vaccines by treating the toxin or vaccine with a tannin, especially a condensed tannin derived from fruit of the genus Diospyros, particularly the persimmon, without loss of the desired antigenicity.
Claims14
A method for the inactivation of a microbial toxin or the attenuation of a vaccine, which comprises contacting the toxin or including in the vaccine, an effective amount of tannin derived from fruit of the genus Diospyros.
A method for the inactivation of a microbial toxin or the attenuation of a vaccine, which comprises contacting the toxin or including in the vaccine an effective amount of a condensed tannin derived from fruit of the genus Diospyros, having the following properties:
- (a) a pale-brown powder in the freeze-dried state;
- (b) soluble in water;
- sparingly soluble in methanol, ethanol, pyridine, acidic acid;
- insoluble in diethyl ether, ethyl acetate, chloroform;
- (c) aqueous solution gives a dark blue coloration with ferric chloride and a brown coloration with lead acetate;
- (d) an infra-red absorption spectrum in liquid paraffin, having main absorption bands at 3400, 1615, 1530, 1380, 1200, 1100 and 1040 cm.sup.-1 ;
- (e) an ultra-violet absorption spectrum measured in an aqueous solution, having an absorption maximum at about 275 nm;
- (f) a chromatogram having a single peak in a gel filtration using a strongly acidic styrene-divinylbenzene copolymer sulphonate resin and water as a mobile phase;
- and
- (g) an ultra-centrifuge Schlieren pattern substantially as shown in FIG. 4 herein before at about 46,633 revs/min for 26 minutes;
- or a salt thereof.
A pharmaceutical composition containing a microbial antigen treated with an effective amount of tannin derived from fruit of the genus Diospyros.
Dependencies are inferred from the claim text.