Immunoassay with test strip having antibodies bound thereto
AssigneeAB Kabi
InventorsAnders O. Grubb, Ulla C. Glad
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Abstract
A test strip for carrying out immunoassays. The test strip is a bibulous carrier to which antibodies are bound. The immunoassay is carried out by wetting the test strip with the aqueous solution containing a suspected antigen. The test strip is then contacted with an aqueous solution containing labeled antibodies.
Claims9
In a method of immunochemical quantification, the improvement which comprises wetting a diagnostic test strip consisting essentially of a porous, capillarity-possessing carrier material having antibodies covalently bound to it, with an aqueous sample containing the antigen to be quantified, allowing the capillary migration to take place, and then assaying the antigen-containing area of said diagnostic test strip by wetting it with antibodies in an aqueous vehicle, said antibodies being bound to a water-soluble fluorescent color indicating compound or to an enzyme that catalyzes a color-developing reaction.
A diagnostic test device useful for immunochemical quantification, which is substantially a carrier strip comprising a silica-modified micro-porous polymer having finely divided silica substantially uniformly embedded in a permeable, continuous polymeric matrix selected from the group consisting of polyvinyl chloride, vinyl chloride-propylene copolymer, and vinyl chloride vinyl acetate copolymer, said strip having antibodies bound to it.
Dependencies are inferred from the claim text.