Insolubilized antibody
AssigneeDainippon Pharmaceutical Co., Ltd.
InventorsShigeru Kurooka, Noriyuki Sunahara
Life of this patent
Estimating the term…
Abstract
An insolubilized antibody suitable for an enzyme immuno assay or radio immuno assay, which has characteristic infrared absorptions at around 1,040 cm.sup.-1, 1,540 cm.sup.-1 and 1,640 cm.sup.-1 and is prepared by chemically binding an antibody to cell wall debris of bacteria or yeasts whose shape is globular or rod-like. There is further disclosed an enzyme immuno assay or radio immuno assay using the insolubilized antibody and a kit containing the insolubilized antibody.
Claims24
An insolubilized antibody for an enzyme immuno assay or radio immuno assay, which has characteristic infrared absorptions at around 1040 cm.sup.-1, 1540 cm.sup.-1 and 1640 cm.sup.-1 comprising said antibody chemically bonded to cell wall debris of bacteria or yeasts whose shape is globular or rod-like.
A kit for an enzyme immuno assay or radio immuno assay, which comprises the following two components:
- Component A:
- an insolubilized antibody which has characteristic infrared absorptions at around 1040 cm.sup.-1, 1540 cm.sup.-1 and 1640 cm.sup.-1 and comprises an antibody chemically bonded to cell wall debris of bacteria or yeasts whose shape is globular or rod-like, and
- Component B:
- an enzyme labeled antigen or a radio isotope labeled antigen.
In a method for conducting an enzyme immuno assay or radio immuno assay, which comprises subjecting an enzyme-or radio isotope-labeled antigen, an unlabeled antigen and an antibody to a competitive antigen-antibody reaction in a buffered solution, separating the enzyme- or ratio isotope-labeled antigen bound to the antibody from the free labeled antigen, and determining the amount of the unlabeled antigen by measuring an enzyme activity or radio activity of either the labeled antigen bound to the antibody or the free labeled antigen, the improvement wherein the antibody is an insolubilized antibody which has characteristic infrared absorptions at around 1040 cm.sup.-1, 1540 cm.sup.-1, and 1640 cm.sup.-1 and comprises an antibody chemically bonded to cell wall debris of bacteria or yeasts whose shape is globular or rod-like.
Dependencies are inferred from the claim text.