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US 4,160,818 AGranted
Fluorimetric immunoassay for diphenylhydantoin
AssigneeTechnicon Instruments Corporation
InventorsDavid S. Smith, Adrienne R. McGregor
Life of this patent
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Abstract
Substituted hydantoin drugs (for example phenytoin) and tricyclic antidepressant drugs (for example nortriptyline) are assayed by mixing them with a fluorescent labelled compound and antibodies, and measuring the fluorescence of the mixture. The assay does not necessitate any separation step and may be effected by continuous-flow techniques.
Claims10
A method of assaying a biological fluid sample for a hydantoin or a closely related hydantoin structured compound, said method comprising the steps of:
- a. forming a mixture of said biological fluid sample containing said compound to-be-assayed with:
- (1) anti-compound antibodies, and (2) a fluorescent-labelled substituted compound, said substituted compound generally having little or no resemblance to said compound to-be-assayed prior to labelling but, having sufficient similarity to said compound to-be-assayed after labelling, so as to be bindable with said anti-compound antibodies, said anti-compound antibodies binding both with said compound to-be-assayed and said fluorescent-labelled substituted compound;
- and
- b. determining the amount of said compound in the fluid sample by measuring the fluorescence of said fluorescent-labelled substituted compound.
1 more element
Dependencies are inferred from the claim text.